Babu Balakrishnan

Research Associate

Mitra Biotech Pvt Ltd.,

November 2013 to Actually working here

? Processing of various types of tumor tissue for the explants culture
? Conducts Hematoxilin & Eosin (H&E) staining procedure to find the cancer tissue morphology and to grade the percentage of tumor cells.
? Preparation of Rosewell Park Memorial Institute (RPMI) medium, Dulbecco?s Minimal Eagle?s Medim (DMEM), Dulbecco?s Phosphate Buffered Saline (DPBS) with antibiotic for the tumor tissue culture
? Writing of various Standard Operating Procedures (SOPs)
? Conducts various biochemical assays f

Associate Research Officer

Christian Meidcal College

October 2011 to October 2013

? Isolation of DNA from various clinical samples (buffycoat, sputum, Mycobacterium tuberculosis culture, using Qiagen kit
? Amplification of DNA from the extracted DNA using conventional PCR (Nested & Non-nested)
? Genotyping of Cryptosporidium sp using Restriction Fragment Length Polymorphism (RFLP)
? Identification of DNA from final PCR product using Agarose Gel Electrophoresis & Gel documentation
? Chip based electrophoresis (MultiNA) an automated electrophoresis detection system

Research Fellow

Sri Narayani Hospital & Research Center

April 2009 to July 2011

? Isolation of DNA from various clinical samples (buffycoat, sputum, Mycobacterium tuberculosis culture) using Qiagen kit
? Amplification of DNA from the extracted DNA using conventional PCR (Nested & Non-nested)
? Identification of DNA from final PCR product using Agarose Gel Electrophoresis & Gel documentation.
? Processing of clinical sputum sample to detect the Mycobacterium tuberculosis
? Separation of Buffy coat from the clinical blood samples to isolate various Microorganism.

Senior Demonstrator

Christian Medical College

April 2008 to March 2009

Assisted reproductive techniques such as: Semen analysis, Sperm & media preparation for Intrauterine insemination (IUI), In-vitro Fertilization (IVF), Intra-cytoplasmic Sperm Injection (ICSI), and identification of sperm from Percutaneous Sperm Aspiration (PESA) & Testicular Sperm Aspiration (TESA)

Medical Technologist

Microlabs Institute of Research & Technology

July 2006 to March 2008

? Routine medical laboratory techniques, such as: Microbiological, Serological, Biochemical, etc.,
? Preparation various reagents and buffers to conduct different type of assays.

M.Sc., Applied Microbiology

July 2004 to June 2006

Career Goals

To benefit an organization that can use an experienced medical lab technician/technologist with skills related to Cancer Biology, Molecular Biology, Serology, Assisted Reproductive Techniques and other areas along with proven communication instructional, research, and problem-solving abilities.

Industries of interest

Biotechnology Laboratories Health

Areas of interest

Quality Health / Pharmacy Laboratories

Availability to travel and work outside the city

Yes

Interests and hobbies